Sajeev Kumar B
Jawaharlal Nehru Technological University, India
Title: Lipid complexed nanocrystals - A safe approach in development of stable nanoparticulates (in-vitro and in-vivo) for effective drug delivery
Biography
Biography: Sajeev Kumar B
Abstract
Nanoparticulates (NPs) are developed with an aim to improve the solubility, targetability and bioavailability of a drug. Lipid Complexed Nanocrystals (LNCs) are novel and unique nanoparticulates off ering high solubility and stability both in vitro and in vivo, hence making it more sustain to body fl uids (electrolytes). In vitro instability (particle aggregation) of NPs may decrease its functional behavior in vivo leading to decreased bioavailability. Development of functional NCs requires modifi cation of its surface properties in sequence to make them clinically more acceptable. Th e study aim to develop Glimepiride NCs using PEG 20000 by nanonization (precipitation) and stabilize them (both in vitro and in vivo) by complexation using Phospholipon 90 G (P 90G). Particle and solid state characterization studies were performed on NCs before and aft er complexation using photon correlation spectroscopy (PCS) and X-ray diff raction spectrometry (PXRD), differential scanning calorimetry (DSC), scanning electron spectroscopy (SEM) and fourier transform infrared spectroscopy (FTIR). In vivo drug targeting effi cacy of LNCs was studied on pancreas of Male Wistar rats using HPLC. Th e particle and solid state characterization results show improved stability (decreased aggregation) with no change in drug properties aft er complexation. In vivo results on optimized LNCs show similar drug concentration in pancreas of rat as that of pure drug. AUC was signifi cantly higher aft er 1 h signifying better in vivo stability. It can be concluded that in vitro and in vivo stability of NCs could be achieved by a complexation using P90 G. Th e possible outcome of these studies could result in development and delivery of stable and safe nanoproducts in the treatment of diabetes.