Meliha Ekinci
Ege University, Turkey
Title: Cell incorporation studies with 99mTc labeled methotrexate loaded chitosan nanoparticles for breast cancer diagnosis
Biography
Biography: Meliha Ekinci
Abstract
The aim of this study is to evaluate newly developed radiopharmaceutical’s (99mTc-MTX-CSNPs) incorporation to human breast cancer (MCF-7) and human keratinocyte (HaCaT) cell lines for breast cancer diagnosis. For this purpose, methotrexate (MTX) loaded chitosan nanoparticles (CSNPs) were prepared by ionic gelation process, storaged during 6 months and evaluated in terms of particle size, polydispersity index value and zeta potential. Produced MTX-CSNPs were radiolabeled by 99mTc with high labeling efficiency (>90%). Then, newly developed radiopharmaceutical’s Target/Non Target ratio was investigated with cell culture studies by using MCF-7 and HaCaT cell lines. Results demonstrated that the incorporation of 99mTc-MTX-CSNPs in breast cancer cells was found about 2-times higher than normal cells. So, 99mTc-MTX-CSNPs might be used for human breast cancer diagnosis in nuclear medicine patients. (The authors thank to Ege University, Scientific Research Projects Comission (Project number: 14/ECZ/037) and Aliye Uster Foundation for financial supports.)