Asha S. Patel
Anand Pharmacy College, India
Title: QbD based systematic development of nanoemulsion formulation containing Boswellia serratta extract with improved biopharmaceutics performance for anti-inflammatory properties
Biography
Biography: Asha S. Patel
Abstract
The current studies entail systematic development, optimization and evaluation (in vitro, ex-vivo and in vivo) of the nanoemulsion based formulation employing rationale of formulation by design (FbD) and multivariate mathematical modelling approach to improve the solubility and in turn bioavailability for enhancing permeation of Boswellic acids in skin layers. Nanoemulsion system comprising of Isopropyl myristate (oily solubilizer), Tween 80 (surfactant) and Transcutol P (co-surfactant) was developed using Simplex lattice mixture experimental design to optimize mixture components effect on critical responses (viz. Y1 Droplet size and Y2 In- vitro cumulative drug permeation). Partial least square regression analysis operates through Excel STAT to influence collinearity of mixture composition on permeation behavior of drug from the nanoemulsion. The size of droplets and permeability coefficients showed good correlation by partial least square regression plots. TEM study confirmed morphological behavior of nanodroplets. The promising nanoemulsion was incorporated into hydrogel using Carbopol 940 for ease of topical application. The optimized formulation was assessed for in-vitro permeation study using dialysis membrane and ex vivo skin permeation using rat abdominal skin. About 3.25 fold increase in flux was seen with nanoemulsion, while nanogel showed 1.45 fold increase in flux compared to carbopol gel with enhancement ratio 4.57 and 1.59 respectively. The permeation data analysis corroborated greater drug permeation and in vivo pharmacodynamic study through Carrageenan induced rat paw edema model demonstrated a pronounced anti-inflammatory effect. Our study illustrated scientific and statistical evidence for potential of developed nanoemulsion with improved biopharmaceutics performance as possible alternative to traditional topical formulations.