Anamika Singh
Govt. Model Science College, India
Title: In-vivo and In-vitro characterization and release potential of 1- B-arabinofuranosylcytosine from casein coated superparamagnetic nanostructure for magnetic drug targeting
Biography
Biography: Anamika Singh
Abstract
Motivation:-Cancer is a group of diseases involving abnormal cell growth with the potential to spread to other parts of the body. Chemotherapy is a type of cancer treatment that uses drugs to destroy cancer cells. Thus realizing the need to overcome complexities involved in treating complex diseases motivated the author to design casein coated iron oxide nanoparticles (CCIONPs) crosslinked with glutaraldehyde for achieving efficient MDT. Method: - In order to design casein nanoparticles (CCIONPs) the microemulsion method was adopted and for impregnation of iron oxide nanoparticles into the bulk of casein matrix an in situ co precipitation method was used. In order to characterize nanoparticles FTIR, XPS, TEM, SEM, VSM, Mossbauer, zeta potential, in-vitro cytotoxicity test were studied. In order to investigate the drug release behaviour of drug loaded CCIONPs the magnetic fields of strength 1000G, 1500G, 2000G, 3000G, 5000G and 5500G were applied. The nanoparticles were loaded with cytarabine and its controlled release was investigated drug loading, chemical architecture of the nanocarriers, and nature of release media. In vivo analysis was perfomed on mice model and different cell lines. Results: - FTIR analysis confirmed homogenous deposition of iron oxide and subsequent formation of CCIONPs. The drug loading efficiency of CCIONPs, drug content and in vitro drug release profiles may be measured by ultraviolet spectrophotometer at λmax 254. It was found to have better payload, in vitro release profile characteristic and better targeting to RES organs. Conclusion: - Glutaraldehyde crosslinked casein coated iron oxide nanoparticles CCIONPs form a swelling controlled drug release system, which effectively delivers cytarabine in the presence and absence of magnetic field via diffusion controlled pathway. Thus, the prepared nanoparticles showed potential to provide a possible option for magnetically targeted delivery of anticancer drugs.